“The petals and thorns” of ROSE (rapid on‐site evaluation)

  title={“The petals and thorns” of ROSE (rapid on‐site evaluation)},
  author={Gilda da Cunha Santos and Hyang Mi Ko and Mauro Ajaj Saieg and William R. Geddie},
  journal={Cancer Cytopathology},
The small samples obtained by these procedures can be used for diagnosis based on morphologic criteria alone and also, in many instances, for special ancillary studies, including molecular analysis. The inherent challenges involved in handling and processing limited samples in such a way as to allow multiple studies have triggered changes in the preanalytical phase and generated new protocols to maximize tissue/cell retrieval. 5 ROSE has been advocated as an effective way to ensure that samples… 

Specimen acquisition: ROSEs, gardeners, and gatekeepers

  • A. Kraft
  • Medicine
    Cancer cytopathology
  • 2017
Current rapid on‐site evaluation practice patterns in different settings are presented, with a special focus on the variety of professionals involved, and challenges to its implementation and how to address them are described.

Imprint cytology: Invaluable technique to evaluate fresh specimens received in the pathology department for lymphoma workup

How fresh specimens received intraoperatively for lymphoma workup are triaged for ancillary studies, such as flow cytometry, florescence in situ hybridization, or molecular analyses based on an interpretation of the touch imprints, is described.

Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA)-from morphology to molecular testing.

Although some evidence concluded that ROSE does not increase the diagnostic efficacy of EBUS-TBNA, other demonstrated that it improves the diagnostic yield of the procedure up to 30%, allows to avoid repetition of additional diagnostic procedures and reduces risk of complications.

ROSE: Alternative for Cancelled and Inconclusive Cytopathologic Examinations, as Well as Professional Training at the UNESP-Botucatu Veterinary Hospital

A case-by-case assessment of inconclusive and cancelled examinations in the Cytopathology Outpatient Clinic at UNESP (HV), Botucatu, Brazil, from 2012 to 2016 is conducted and ascertains the causes.

Management of thyroid cytological material, preanalytical procedures and bio‐banking

Technical aspects (preanalytics) of performing thyroid FNAC, including image guidance and rapid on‐site evaluation, sample collection methods (conventional slides, liquid‐based methods, cell blocks) and storage (bio‐banking).

Two decades of lung cancer through the pages of Cancer Cytopathology

Looking back over 20 years in the annals of Cancer Cytopathology, 3 developments have undoubtedly carried the pulmonary cytopathologist into the modern arena of lung cancer diagnosis, staging, and prognosis.

This ‘Rose’ Has no Thorns—Diagnostic Utility of ‘Rapid On-Site Evaluation’ (ROSE) in Fine Needle Aspiration Cytology

To evaluate the role of ROSE using toluidine blue staining in arriving at a diagnosis in comparison to routine stains, a total of 1500 cases of FNA of palpable swellings from sites like salivary gland, breast, thyroid, lymph node, and soft tissue lesions during a 9-month period were studied.

Advances in Molecular Testing Techniques in Cytologic Specimens.




Diagnostic value and cost‐effectiveness of on‐site evaluation of fine‐needle aspiration specimens: Review of 5,688 cases

On‐site cytopathologic evaluation of FNA specimens is accurate, cost‐effective, and has improved patient care at the institution and could be realized by utilizing on‐site evaluation despite the additional fee due to a higher rate of specimen adequacy.

Diagnostic difficulties and pitfalls in rapid on-site evaluation of endobronchial ultrasound guided fine needle aspiration

The cytomorphology of five EBUS FNA cases is illustrated to address some of the diagnostic challenges witnessed while examining these specimens during ROSE, including the baseline cellularity of the aspirates, the bronchial contamination, the difficulty identifying neoplasms with bland cytology, the wide spectrum of diseases that can occur in the mediastinum with overlapping cytomorphologic features, and the overall unfamiliarity with these types of specimens.

Utility of on-site cytopathology assessment for bronchoscopic evaluation of lung masses and adenopathy.

Investigating the extent to which on-site cytopathology assessment improves diagnostic yield when sampling lung nodules or masses and/or hilar or mediastinal lymphadenopathy by fiberoptic bronchoscopy concluded that diagnostic yield was greater when on- site cy topathology was used to assist FOB evaluation of intrathoracic adenopathy and/ or lung nodule or masses.

Rapid on-site evaluation of transbronchial aspirates in the diagnosis of hilar and mediastinal adenopathy: a randomized trial.

ROSE of transbronchial aspirates from hilar and mediastinal nodes enables avoidance of additional biopsy without loss in diagnostic yield and reduces the complication rate of bronchoscopy.

Should endobronchial ultrasonography be part of the thoracic surgeon's armamentarium?

Clinical Impact of On-Site Cytopathology Interpretation on Endoscopic Ultrasound-Guided Fine Needle Aspiration

On-site cytopathology interpretation improves the diagnostic yield of EUS-guided FNA and EUS centers should allocate resources to cover for on- site cytopATHology evaluation.

Agreement between Rapid Onsite and Final Cytologic Interpretations of EUS-guided FNA Specimens: Implications for the Endosonographer and Patient Management

ROSE of EUS-FNA specimens is highly accurate compared with final cytologic evaluation, and informing patients and their families and referring physicians of a cancer diagnosis significantly reduces physician work load and expedites patient management.

Endobronchial ultrasound-guided transbronchial needle aspiration biopsy is useful evaluating mediastinal lymphadenopathy in a cancer center

EBUS-TBNA biopsy is a sensitive and specific method for evaluating mediastinal lymphadenopathy in patients with lung and other primary tumors and after one year clinical/radiological and histologic follow-up rates are 97%, 99.3%, 96.7% and 99.4%, respectively.

Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration for Lymph Node Staging in Patients with Non-small Cell Lung Cancer in Nonoperable Patients Pursuing Radiotherapy as a Primary Treatment

  • T. NakajimaK. Yasufuku I. Yoshino
  • Medicine
    Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
  • 2010
EBUS-TBNA offers accurate minimally invasive lymph node staging in patients who are candidates for CIRT and can be safely performed with a high diagnostic accuracy before CIRT.

Telecytopathology for immediate evaluation of fine‐needle aspiration specimens

The authors present the experience with on‐site evaluation using telecytopathology for fine‐needle aspiration specimens by a pathologist to obtain adequate samples and provide a preliminary diagnosis.