“GenderPlex” a PCR multiplex for reliable gender determination of degraded human DNA samples and complex gender constellations

@article{Codina2008GenderPlexAP,
  title={“GenderPlex” a PCR multiplex for reliable gender determination of degraded human DNA samples and complex gender constellations},
  author={Anna Esteve Codina and Harald Niederst{\"a}tter and Walther Parson},
  journal={International Journal of Legal Medicine},
  year={2008},
  volume={123},
  pages={459-464}
}
The amelogenin test integrated in most commercial polymerase chain reaction (PCR) multiplex kits is routinely used in the forensic field for gender determination of DNA samples. It has been demonstrated that this test is not entirely reliable. Males with deletions in the homologous amelogenin part on the Y chromosome (AMELY) were erroneously typed as females due to lack of Y-specific amelogenin amplification. Also, primer binding site mutations that result in a failure to amplify the AMELY or… CONTINUE READING