‘Protected DNA Probes’ capable of strong hybridization without removal of base protecting groups

  title={‘Protected DNA Probes’ capable of strong hybridization without removal of base protecting groups},
  author={Akihiro Ohkubo and Rintaro Kasuya and Kazushi Sakamoto and Ken-ichi Miyata and Haruhiko Taguchi and Hiroshi Nagasawa and Toshifumi Tsukahara and Takuma Watanobe and Yoshiyuki Maki and Kohji Seio and Mitsuo Sekine},
  journal={Nucleic Acids Research},
  pages={1952 - 1964}
We propose a new strategy called the ‘Protected DNA Probes (PDP) method’ in which appropriately protected bases selectively bind to the complementary bases without the removal of their base protecting groups. Previously, we reported that 4-N-acetylcytosine oligonucleotides (ac4C) exhibited a higher hybridization affinity for ssDNA than the unmodified oligonucleotides. For the PDP strategy, we created a modified adenine base and synthesized an N-acylated deoxyadenosine mimic having 6-N-acetyl-8… 

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