μ‐Antibody capture elisa for the rapid diagnosis of enterovirus infections in patients with aseptic meningitis

@article{Bell1986AntibodyCE,
  title={$\mu$‐Antibody capture elisa for the rapid diagnosis of enterovirus infections in patients with aseptic meningitis},
  author={Eleanor J. Bell and Ruth A. McCartney and Diane Basquill and A. K. R. Chaudhuri},
  journal={Journal of Medical Virology},
  year={1986},
  volume={19}
}
Between August and November 1985, 45 patients with suspected aseptic meningitis were investigated using conventional virus isolation procedures and the m̈‐antibody capture Coxsackie B IgM enzyme‐linked immunoabsorbent assay (ELISA) test, which is well known to cross‐react with other members of the enterovirus group. An enterovirus was isolated from 22% of patients compared with 67% who were positive in the ELISA test. Not only was the rate of enterovirus detection increased by using this ELISA… 
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60 Citations
Background Citations
8
Methods Citations
2
Results Citations
2

60 Citations

Sensitivity and specificity of mu-capture ELISA for detection of enterovirus IgM.
Enterovirus-specific IgM in the diagnosis of meningitis.
Early diagnosis of enteroviral meningitis by detection of specific IgM antibodies with a solid‐phase reverse immunosorbent test (SPRIST) and μ‐capture EIA
TLDR
Results indicate that the IgM antibody response appears early in the course of aseptic meningitis.
Aspects on the serodiagnosis of enterovirus infections by ELISA
Diagnosis of enteroviral meningitis with IgG‐EIA using heat‐treated virions and synthetic peptides as antigens
TLDR
Two recently developed enzyme immunosorbent assays for the detection of significant titre increases in enteroviral IgG‐antibodies were evaluated as diagnostic tools in 127 etiologically well‐characterized patients with aseptic meningitis and seemed to be a valuable alternative to CFT for the routine diagnosis of enterovirus infection.
Laboratory diagnosis of enteroviral infections of the central nervous system by using a nested RT-polymerase chain reaction (PCR) assay.
Immunoglobulin M capture immunoassay in investigation of coxsackievirus B5 and B6 outbreaks in South Australia
  • P. Goldwater
  • Medicine, Biology
    Journal of clinical microbiology
  • 1995
TLDR
This study demonstrated success of the IgM enzyme immunoassay and the need for diagnostic virology laboratories to look for CBV-6 infection in addition to the other five CBVs.
Comparative evaluation of immunoglobulin M neutralizing antibody response in acute-phase sera and virus isolation for the routine diagnosis of enterovirus infection
TLDR
IgM neutralizing antibody response on acute-phase serum appears to be of limited value in the rapid diagnosis of acute EV infection but may prove useful for the investigation of the wide range of chronic diseases associated with EV.
Early diagnosis of enteroviral meningitis by a solid-phase reverse immunosorbent test and virus isolation.
TLDR
Both SPRIST and virus isolation seem to be valuable for the early diagnosis of enteroviral meningitis, especially in patients with an enterovirus isolate.
Detection of neutralizing igm antibodies in the diagnosis of enterovirus infections
TLDR
The susceptibility of the elderly and the frequency of IgM‐positive sera among adults over age 40 years suggests possible underestimation of enterovirus infections in adults.
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References

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TLDR
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