μ‐Antibody capture elisa for the rapid diagnosis of enterovirus infections in patients with aseptic meningitis

@article{Bell1986AntibodyCE,
  title={$\mu$‐Antibody capture elisa for the rapid diagnosis of enterovirus infections in patients with aseptic meningitis},
  author={Eleanor J. Bell and Ruth A. McCartney and Diane Basquill and A. K. R. Chaudhuri},
  journal={Journal of Medical Virology},
  year={1986},
  volume={19}
}
Between August and November 1985, 45 patients with suspected aseptic meningitis were investigated using conventional virus isolation procedures and the m̈‐antibody capture Coxsackie B IgM enzyme‐linked immunoabsorbent assay (ELISA) test, which is well known to cross‐react with other members of the enterovirus group. An enterovirus was isolated from 22% of patients compared with 67% who were positive in the ELISA test. Not only was the rate of enterovirus detection increased by using this ELISA… 
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References

SHOWING 1-6 OF 6 REFERENCES
Routine use of μ‐antibody‐capture elisa for the serological diagnosis of coxsackie b virus infections
TLDR
A regimen for the routine serological diagnosis of CBV infections was introduced, using the CBV IgM ELISA as a screening test, followed by neutralization tests (NT) to confirm the positive results.
Heterotypic reactions in a radioimmunoassay for coxsackie B virus specific IgM.
  • S. Pugh
  • Biology, Medicine
    Journal of clinical pathology
  • 1984
TLDR
Thirty seven patients with other enterovirus infections were studied, and sera from 15 of these patients reacted in the assay, showing that heterotypic coxsackie B IgM responses occur not only in coxsackedie B virus infections but also in other entervirus infections.