γ-Glutamyl-γ-aminobutyrate hydrolase in the putrescine utilization pathway of Escherichia coli K-12

@inproceedings{Kurihara2006GlutamylaminobutyrateHI,
  title={γ-Glutamyl-γ-aminobutyrate hydrolase in the putrescine utilization pathway of Escherichia coli K-12},
  author={Shin Kurihara and Shinpei Oda and Hidehiko Kumagai and Hideyuki Suzuki},
  year={2006}
}
γ-Glutamyl-γ-aminobutyrate hydrolase (PuuD) was purified and the properties of the enzyme were characterized. The active center of PuuD was identified as Cys-114 by site-directed mutagenesis. The expression of PuuD was induced by putrescine and O2 (substrates of the Puu pathway), while the addition of succinate or NH4Cl (products of the Puu pathway) to the medium reduced the expression of PuuD. The findings that the puuD -deficient strain accumulated γ-glutamyl-γ-aminobutyrate (γ-Glu-GABA) and… CONTINUE READING

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The putrescine Importer PuuP of Escherichia coli K-12.

  • Journal of bacteriology
  • 2009
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