β-d-glucosyl-hydroxymethyluracil: A novel modified base present in the DNA of the parasitic protozoan T. brucei
@article{GommersAmpt1993dglucosylhydroxymethyluracilAN, title={$\beta$-d-glucosyl-hydroxymethyluracil: A novel modified base present in the DNA of the parasitic protozoan T. brucei}, author={J H Gommers-Ampt and Fred van Leeuwen and Antonius L. J. de Beer and Johannes F. G. Vliegenthart and Miral Dizdaroglu and Jeffrey A. Kowalak and Pamela F. Crain and Piet Borst}, journal={Cell}, year={1993}, volume={75}, pages={1129-1136} }
162 Citations
beta-D-glucosyl-hydroxymethyluracil, a novel base in African trypanosomes and other Kinetoplastida.
- Biology, ChemistryMolecular and biochemical parasitology
- 1997
beta-D-glucosyl-hydroxymethyluracil is a conserved DNA modification in kinetoplastid protozoans and is abundant in their telomeres.
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 1998
The evolutionary conservation of J in kinetoplastid protozoans suggests that it has a general function, repression of transcription or recombination, or a combination of both.
Biosynthesis and Function of the Modified DNA Base β-d-Glucosyl-Hydroxymethyluracil inTrypanosoma brucei
- Biology, ChemistryMolecular and Cellular Biology
- 1998
Study of J in T. brucei found that incorporation of bromodeoxyuridine resulted in a 12-fold decrease in J content and caused a partial derepression of silent VSG gene expression site promoters, suggesting that J might strengthen transcriptional repression.
Quantitative Mass Spectrometry-Based Analysis of β-D-Glucosyl-5-Hydroxymethyluracil in Genomic DNA of Trypanosoma brucei
- Biology, ChemistryJournal of The American Society for Mass Spectrometry
- 2014
Results provided direct evidence supporting that JBP proteins play an important role in oxidizing thymidine to form 5-HmdU, which facilitated the generation of dJ, the first report about the application of LC-MS/MS for the quantification of base J.
Identification of the Glucosyltransferase That Converts Hydroxymethyluracil to Base J in the Trypanosomatid Genome*
- BiologyThe Journal of Biological Chemistry
- 2014
The analysis of JGT function confirms the two-step J synthesis model and demonstrates that JGT is the only glucosyltransferase enzyme required for the second step of the pathway.
Site-specific Interactions of JBP with Base and Sugar Moieties in Duplex J-DNA
- Biology, ChemistryThe Journal of Biological Chemistry
- 2002
Examination of molecular interactions that contribute to recognition of the glycosylated base in synthetic DNA substrates using modification interference, modification protection, DNA footprinting, and photocross-linking techniques finds that the two primary requirements for J-DNA recognition include contacts at base J and a base immediately 5′ of J (J-1).
Biosynthesis, and Possible Functions
- Biology
- 2008
The identification and localization of base J in the genome of kinetoplastids, the enzymes involved in J biosynthesis, possible biological functions of J, and J as a potential target for chemotherapy of diseases caused by kinetiplastids are discussed.
The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase
- Biology, ChemistryNucleic acids research
- 2007
Evidence is presented that a protein that binds to J in DNA, the J-binding protein 1 (JBP1), may also catalyze the first step in J biosynthesis, the conversion of thymine in DNA into hydroxymethyluracil and it is proposed that JBP1 is a thymidine hydroxylase responsible for the local amplification of J inserted by JBP2, another putative thymalid hydroxyase.
vidence that J-binding protein 2 is a thymidine hydroxylase catalyzing he first step in the biosynthesis of DNA base
- Biology, Chemistry
- 2009
The genomic DNA of kinetoplastid parasites contains a unique modified base, -d-glucosylhydroxymethyluracil or base J, and two proteins, called JBP 1 and 2, display features of the family of Fe(II)–2-oxoglutarate dependent dioxygenases and are likely to be the enzymes catalyzing the first step in J biosynthesis.
Tandemly repeated DNA is a target for the partial replacement of thymine by beta-D-glucosyl-hydroxymethyluracil in Trypanosoma brucei.
- BiologyMolecular and biochemical parasitology
- 2000
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