β-1,3-Glucanase from Unfertilized Eggs of the Sea Urchin Strongylocentrotus intermedius. Comparison with β-1,3-Glucanases of Marine and Terrestrial Mollusks

@article{Sova200413GlucanaseFU,
  title={$\beta$-1,3-Glucanase from Unfertilized Eggs of the Sea Urchin Strongylocentrotus intermedius. Comparison with $\beta$-1,3-Glucanases of Marine and Terrestrial Mollusks},
  author={V. V. Sova and Nadya Shirokova and Mikhail I. Kusaykin and A S Scobun and Ludmila A. Elyakova and Tatyana N. Zvyagintseva},
  journal={Biochemistry (Moscow)},
  year={2004},
  volume={68},
  pages={529-533}
}
Abstractβ-1,3-Glucanase (Lu) was isolated from unfertilized eggs of the sea urchin Strongylocentrotus intermedius. A comparative study of some properties of β-1,3-glucanase Lu and β-1,3-glucanases with different action types—endo-β-1,3-glucanase from crystalline style of the marine mollusk Spisula sachalinensis (LIV) and exo-β-1,3-glucanase from the terrestrial snail Eulota maakii (LII)—was performed. It was found that β-1,3-glucanase Lu hydrolyzes laminaran with a high yield of glucose in the… 

Figures and Tables from this paper

The effects of brown alga proteins and polysaccharides on egg fertilization and the development of embryos of the sea urchin Strongylocentrotus intermedius A. Agassiz, 1863
TLDR
Preadministration of polysaccharides completely neutralized the inhibitory effects of the proteins on developing embryos and it was shown that a water–ethanol extract of S. cichorioides, derived I1 and I2 proteins, and fucoidans has a contraceptive effect.
The Effect of Metabolites from the Brown Alga Saccharina cichorioides (Miyabe) on the Feeding Habits, Fertilization, and Embryonic Development of the Sea Urchin Strongylocentrotus intermedius A. Agassiz, 1863
The substances contained in the aqueous–ethanol and aqueous extracts from freshly collected and destroyed brown algae Saccharina cichorioides in their second vegetation season and from excrement of
Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family
TLDR
The biochemical characterization and crystal structure of this novel GH may enable discovery of other β-1,2-glucanases and represent a critical advance toward elucidating structure-function relationships of GH enzymes.
Purification and partial characterization of a novel β-1,3-glucanase from the gut of sea cucumber Stichopus japonicus
Abstract For the first time, a β-1,3-glucanase was isolated from the gut of sea cucumber. The enzyme was purified to homogeneity by ammonium sulfate precipitation, ion exchange chromatography with
Purification and characterization of a novel β-1,3-glucanase from Arca inflata and its immune-enhancing effects.
TLDR
The novel β-1,3-glucanase exhibited immune-enhancing effects via significantly increasing the phagocytic activity of macrophages and inducing the release of nitric oxide, tumor necrosis factor α, and interleukin-6 in RAW2647 cells.
Laminarans and 1,3-β-D-glucanases.
TLDR
This review focused on information about sources, structure and properties of laminarans and 1,3-β-D-glucanases, methods of obtaining and structural elucidation of laminationarans, and biological activity of laminae and products of their enzymatic transformation.
beta-1,3-Glucanase gene expression in low-hydrated seeds as a mechanism for dormancy release during tobacco after-ripening.
  • G. Leubner-Metzger
  • Biology, Medicine
    The Plant journal : for cell and molecular biology
  • 2005
TLDR
De novo gene expression is proposed to be a novel molecular mechanism for the release of coat-imposed dormancy during oilseed after-ripening.
Marine glycosyl hydrolases in the hydrolysis and synthesis of oligosaccharides
TLDR
Applications of these biocatalysts for vegetal waste treatment in recovering useful materials, for structural identification and for preparation of target materials from new purified polysaccharides, for the synthesis or modification of food‐related compounds and for glycobiology related studies are considered.
Stability of prebiotic, laminaran oligosaccharide under food processing conditions
Prebiotic stability tests on laminaran oligosaccharide under food processing conditions were urgently performed to determine the ability of prebiotics deal with processing. Laminaran, oligosaccharide
Antivirals against animal viruses
TLDR
This review describes natural and synthetic antivirals in chronological order, with an emphasis on natural compounds, even when their mechanisms of action are not completely understood, that could serve as the basis for future development of novel and/or complementary antiviral treatments.

References

SHOWING 1-10 OF 17 REFERENCES
Comparative studies on β-glucan hydrolases: Isolation and characterization of an exo(1→3)-β-glucanase from the snail, Helix pomatia
TLDR
An exo-β-glucan hydrolase, present in the digestive juice of the snail, Helix pomatia, has been purified to homogeneity by chromatography on Bio-Gel P-60, Sephadex G-200, DeAE-cellulose, and DEAE-SephadeX to show broadness of its substrate specificity.
Two antigenically distinct forms of β-1,3-glucanase in sea urchin embryonic development
TLDR
The antibody was used to demonstrate that the form of β-1,3-glucanase present following gastrulation is antigenically distinct from the egg form, suggesting that it may function in larval digestion.
Hydrolase and transferase activities of the beta-1,3-exoglucanase of Candida albicans.
TLDR
The kinetics of exo-beta-1,3-glucanase of Candida albicans and the predicted substrate-binding site for up to five sugar residues are consistent with a recent structural analysis of the enzyme- binding site.
Purification and properties of an exo-β-d-1,3-glucanase from sea urchin eggs
TLDR
An exo-β-d-1,3-glucanase has been purified 200-fold from unfertilized eggs of the sea urchin Strongylocentrotus purpuratus by DEAE-cellulose chromatography, and it is speculated that the enzyme is involved in the early events of fertilization associated with breakdown of cortical granules.
Stage-specific expression of β-1, 3-glucanase in sea urchin embryos and hybrids
TLDR
The results indicate the two forms of β-1, 3-glucanase (egg and embryonic) are expressed independently and in a stage-specific manner, suggesting they are not functionally equivalent.
Purification and some properties of beta-1,3-glucan glucanohydrolase from the crystalline style of bivalvia, Spisula sachalinensis.
TLDR
The enzyme hydrolyzed laminarin; it was not active towards glucobioses and other sugars; it gave rise to glucose and laminaribiose on lengthy hydrolysis.
Some aspects of the specificity and action pattern of -I,3-glucan glucanohydrolase from Spisula sachalinensis.
TLDR
The study of some aspects of the specificity and action pattern of laminarinases from Spisula sachalinensis has been carried out, and both enzymes can probably be ascribed to endo-β-(1→3)-glucan glucanohydrolases.
A β-1,3-glucan hydrolase from Nicotiana glutinosa: I. Extraction, purification and physical properties
Abstract 1. 1.|A β-1,3-glucan hydrolase from the leaves of Nicotiana glutinosa has been purified by chromatography on DEAE-cellulose and Bio-Gel P-150, followed by isoelectric focusing. A 280-fold
Some properties of A β-1,3-glucanase from rye☆
TLDR
Molecular-sieve chromatography of an extract from ungerminated rye indicated the presence of enzymes which hydrolysed cellobiose, laminaribiose and the β-glucans cellodextrin,Laminarin and barley β- glucan, and the in vivo role of the enzyme was discussed.
The Hydrolytic Mechanism of an Exo-β-(1 → 3)-d-glucanase
Abstract The hydrolytic mechanism of an exo-β-(1 → 3)-d-glucanase was investigated. The enzyme quantitatively inverts anomeric configuration producing α-d-glucose, and will not catalyze
...
1
2
...