β‐Glucosidase Catalyzing Specific Hydrolysis of an Iridoid β‐Glucoside from Plumeria obtusa

@article{Boonclarm2006GlucosidaseCS,
  title={$\beta$‐Glucosidase Catalyzing Specific Hydrolysis of an Iridoid $\beta$‐Glucoside from Plumeria obtusa},
  author={Doungkamol Boonclarm and Thakorn Sornwatana and Dumrongkiet Arthan and Palangpon Kongsaeree and Jisnuson Svasti},
  journal={Acta Biochimica et Biophysica Sinica},
  year={2006},
  volume={38},
  pages={563-570}
}
An iridoid β-glucoside, namely plumieride coumarate glucoside, was isolated from the Plumeria obtusa (white frangipani) flower. A β-glucosidase, purified to homogeneity from P. obtusa, could hydrolyze plumieride coumarate glucoside to its corresponding 13-O-coumarylplumieride. Plumeria β-glucosidase is a monomeric glycoprotein with a molecular weight of 60.6 kDa and an isoelectric point of 4.90. The purified β-glucosidase had an optimum pH of 5.5 for p-nitrophenol (pNP)-β-D-glucoside and for… 

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