α-5 Laminin Synthesized by Human Pluripotent Stem Cells Promotes Self-Renewal

@inproceedings{Laperle20155LS,
  title={α-5 Laminin Synthesized by Human Pluripotent Stem Cells Promotes Self-Renewal},
  author={Alex Laperle and Cheston Hsiao and Michael Lampe and Jaime Mortier and Krishanu Saha and Sean P Palecek and Kristyn S. Masters},
  booktitle={Stem cell reports},
  year={2015}
}
Substrate composition significantly impacts human pluripotent stem cell (hPSC) self-renewal and differentiation, but relatively little is known about the role of endogenously produced extracellular matrix (ECM) components in regulating hPSC fates. Here we identify α-5 laminin as a signature ECM component endogenously synthesized by undifferentiated hPSCs cultured on defined substrates. Inducible shRNA knockdown and Cas9-mediated disruption of the LAMA5 gene dramatically reduced hPSC self… CONTINUE READING
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