Corpus ID: 194961413

Établissement d'une lignée de souris transgéniques exprimant l'isoforme p35 de la chaîne invariante et développement d'un anticorps polyclonal spécifique

  title={{\'E}tablissement d'une lign{\'e}e de souris transg{\'e}niques exprimant l'isoforme p35 de la cha{\^i}ne invariante et d{\'e}veloppement d'un anticorps polyclonal sp{\'e}cifique},
  author={Catherine M{\'e}nard},
MHC class II are the predominant molecules implicated in the CD4+ T cell antigenic peptide presentation. The MHC class II functionality needs the presence of a chaperone proteîn called the invariant chain (Ii). The MHC class II assembly and maturation is facilitated by de presence ofli. The cytoplasmic tail ofli contains a targeting motifthat is important for the migration of the complex to the endosomes where the peptide exchange takes place. In humans, the most important isoforms of Ii are… Expand


The invariant chain is required for intracellular transport and function of major histocompatibility complex class II molecules
The role of Ii chain is defined as a chaperone that assists class II during folding, assembly, and transport and causes them to be recognized as "misfolded" and retained in the same compartments as bona fide misfolded proteins. Expand
Reconstitution of invariant chain function in transgenic mice in vivo by individual p31 and p41 isoforms.
Low level expression of p31 or p41 is not sufficient for rescuing high levels of cell surface class II expression, however, low levels of the typical compact dimer conformation indicative of tight peptide binding are observed, and both isoforms participate in class II folding and assembly. Expand
Glycosylation Signals That Separate the Trimerization from the MHC Class II-binding Domain Control Intracellular Degradation of Invariant Chain*
The data suggest that N-linked glycosylation of Ii hinders premature proteolysis in nonacidic vesicles resulting in Ii degradation in acidic MHC class II-processing compartments. Expand
The requirement for DM in class II-restricted antigen presentation and SDS-stable dimer formation is allele and species dependent
The results suggest that DM may modulate rather than play a requisite role in I-Ad- restricted antigen presentation and SDS-stable dimer formation and that dependency on DM may be allele or species specific. Expand
Invariant chain, a chain of command.
It is proposed that the roles of Ii as a chaperone and as a signaling molecule are intertwined and shows remarkable similarities to the function of proteins processed by RIP. Expand
Functional Characterization of a Lysosomal Sorting Motif in the Cytoplasmic Tail of HLA-DOβ*
The results demonstrate that lysosomal expression of the DO-DM complex is mediated primarily by the tyrosine-based motif of HLA-DM and suggest that the DOβ-encoded motif is involved in the fine-tuning of the intracellular sorting. Expand
The MHC class II beta chain cytoplasmic tail overcomes the invariant chain p35-encoded endoplasmic reticulum retention signal.
Interestingly, these results clearly demonstrate for the first time that DP and DQ isotypes can also overcome the retention motif of Iip35 through a mechanism involving their very distinctive polymorphic beta chain cytoplasmic tails. Expand
Mini‐review: Specificity and expression of CIITA, the master regulator of MHC class II genes
CIITA is by a long stretch the single most important transcription factor for the regulation of genes required for MHCII‐restricted antigen‐presentation. Expand
Discovering the role of the major histocompatibility complex in the immune response.
  • H. Mcdevitt
  • Biology, Medicine
  • Annual review of immunology
  • 2000
The discovery that genes in the major histocompatibility complex (MHC) play an important role in the immune response depended on the chance interaction of several unrelated events. The first, andExpand
Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens.
There appears to be a free pool of the invariant chain and its processed form that is not found associated with DR alpha and beta chains, and this processing pattern was seen in peripheral blood lymphocytes, freshly purified monocytes, and in an interleukin 2-dependent continuous T cell line that expressed HLA-DR. Expand