[luxAB genes as marker for detecting Rhizobium fredii HN01 nodulation functions].

A suicide plasmid pHNC3 which contains Tn5-luxAB was transferred into Rhizobium fredii HN01 by the help of pRK2013. Then Tn5-luxAB inserted on the genome of HN01 and gave luminescence activity. The luminescence colonies were picked up and the Eckhardt gel was performed for plasmids profile detection. The location of Tn5-luxAB on the genome was determined… CONTINUE READING