[Western blot technique standardization of the diagnosis of human fasciolosis using Fasciola hepatica excreted-secreted antigens].


OBJECTIVES To evaluate the performance of the enzyme-linked immunoelectrotransfer blot assay (EITB, Western blot) using excretory/secretory antigens from adult forms of Fasciola hepatica (Fh E/S Ag) for the diagnosis of human fasciolosis. MATERIALS AND METHODS Antigens were obtained after 18 hours of incubation in culture medium Minimum Essential Eagle… (More)


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