• Biology, Medicine
  • Published in
    Sheng wu gong cheng xue bao…
    2004

[Using green fluorescent protein as a reporter to monitor elimination of selectable marker genes from transgenic plants].

@article{Jia2004UsingGF,
  title={[Using green fluorescent protein as a reporter to monitor elimination of selectable marker genes from transgenic plants].},
  author={Hong-ge Jia and Ling-Fei L{\"u} and Yong-qi Pang and Xiao-ying Chen and Rong-xiang Fang},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2004},
  volume={20 1},
  pages={
          10-5
        }
}
In genetic modification of plants, once the transformants are obtained, selection markers are no longer required in mature plants. At present, the Cre/lox site-specific recombination system is most widely used to eliminate the selectable marker genes from the transgenic plants. In this study, attempt was made to favour the selection of marker-free plants in the re-transformation method. Green fluorescent protein (GFP) can be directly visualized in living cells, tissues or organisms under UV… CONTINUE READING