[Transformation of the cells of transplantable teratocarcinoma CBA9H6 by a bacterial dihydrofolate reductase gene].

@article{Tomilin1987TransformationOT,
  title={[Transformation of the cells of transplantable teratocarcinoma CBA9H6 by a bacterial dihydrofolate reductase gene].},
  author={Nikolai Viktorovich Tomilin and A V Titomirov and L I Stepan'ian and Pavel A. Dyban},
  journal={Tsitologiia},
  year={1987},
  volume={29 12},
  pages={1385-91}
}
A plasmid containing the bacterial gene of methotrexate-resistant dihydrofolate reductase (dhfr), under the control of early SV40 promoter, was introduced into murine teratocarcinoma CBA9H6 cells. From the whole pool of teratocarcinoma cells, which survived after transient methotrexate selection in vivo, the individual cells were isolated to give rise to 15 clones of tumors. Six of the 15 clones displayed nucleotide sequences of the original vector containing pBR322 sequences and the early SV40… CONTINUE READING

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