[Transfection of articular chondrocytes with PcNDA3-hBMP3 and its stable expression].

Abstract

OBJECTIVE To explore the possibility of stable expression of PcDNA3-hBMP3 in cultured articular chondrocytes of rabbit. METHODS PcDNA3-hBMP3 was constructed using gene clone technique and recombined DNA technique. With the help of profectamine, the cultured articular chondrocytes were transfected with PcDNA3-hBMP3, and the evidence of successfully stable… (More)

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