[The isolation and immunochemical study of Leishmania antigens].

The authors describe a procedure for producing the antigen by using a promastigotic suspension of the high-virulent inoculative strain Leishmania major. A Leishmania culture medium was improved to enhance the yield of parasites up to 1-3 billion cells in a flask. The grown culture was washed off with isotonic sodium chloride solution, from the medium… CONTINUE READING