[The effect of middle ear effusion on enzymatic digestion of DNA in middle ear effusions of chronic otitis media with effusion].


OBJECTIVE To determine whether or not the bacterial DNA which was detected by PCR comes from viable bacteria. METHOD Observing the affection of middle ear effusion (MEE) on DNA viscosity and enzymatic digestion of DNA. RESULT The middle ear effusion and DNA are stable and DNase 1 rapidly digests DNA. The effusion does not seem to degrade DNA. The middle… (More)


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