[The effect of fixation, dehydration and polymethacrylate imbedding on the results of immuno- and enzymehistochemical studies on lymphatic tissue and bone marrow].

@article{Hantschick1988TheEO,
  title={[The effect of fixation, dehydration and polymethacrylate imbedding on the results of immuno- and enzymehistochemical studies on lymphatic tissue and bone marrow].},
  author={M Hantschick and Eduard Wolf and G W Dominok},
  journal={Acta histochemica. Supplementband},
  year={1988},
  volume={35},
  pages={165-77}
}
The influence of formaldehyde fixation, dehydration and polymethacrylate embedding on the results of immunofluorescence staining of immunoglobulin and enzymehistochemical demonstration of hydrolases, especially acid phosphatase and alpha-naphthylacetate esterase in human tonsils and bone marrow were studied. The best results were achieved by using 2% formaldehyde and 5% sucrose in 0.02 M phosphate buffer (ph 7.4) for fixation and ethyleneglycol monobutylether for dehydration. The procedures… CONTINUE READING