• Corpus ID: 21513696

[The content of tyrosine in rat luteal cell and the changes caused by hCG, cAMP and progesterone].

@article{Zhang1989TheCO,
  title={[The content of tyrosine in rat luteal cell and the changes caused by hCG, cAMP and progesterone].},
  author={Q. Zhang and X N Wang and E W Gao and C P Cheng},
  journal={Sheng li xue bao : [Acta physiologica Sinica]},
  year={1989},
  volume={41 1},
  pages={
          70-5
        }
}
The ovaries taken from the immature female rats primed with PMSG-hCG were digested with collagenase-DNAase solution to obtain the luteal cell suspensions. Luteal cells were then incubated with different doses of hCG, cAMP or progesterone for 1.5 hours. The contents of tyrosine in luteal cell suspensions were determined by thin layer chromatogram using dual-wavelength chromatogram scanner. It was found that the content of tyrosine in rat luteal cell suspensions was 1.41 + 0.24 nmol/L/10(6) cells…