[The construction of yeast two-hybrid method in the protein-interactions and its significance in hepatic metastasis of colorectal carcinoma].

@article{Zuo2004TheCO,
  title={[The construction of yeast two-hybrid method in the protein-interactions and its significance in hepatic metastasis of colorectal carcinoma].},
  author={Fu-yi Zuo and Shi-yong Li and Ping An and Bo Yu and Hui-yun Cai},
  journal={Zhonghua wai ke za zhi [Chinese journal of surgery]},
  year={2004},
  volume={42 11},
  pages={672-4}
}
OBJECTIVE To construct the yeast two-hybrid system, and screen the proteins which interact with FasL, and investigate the relationship of FasL and hepatic metastasis of colorectal carcinoma. METHODS We have cloned the FasL gene into the pGBKT7 vector as the bait, then screened the fetal liver cDNA library, and have got a series of specific proteins that interact with FasL protein. Using the bioinformatics, we analyzed the interacting proteins in the mechanism of hepatic metastasis of… CONTINUE READING