[The construction and expression of 6B11scFv anti-idiotypic antibody and human granulocyte-macrophage-colony stimulating factor in Escherichia coli].

Abstract

OBJECTIVE To enhance the antigenicity of an anti-idiotypic single chain antibody 6B11 mimicking ovarian cancer antigen. METHODS Using DNA recombinant technique, a recombinant fusion protein expression vector was constructed. This vector linked 6B11scFv cDNA and human GM-CSF with a linker. Protein productions were induced with the temperature change in E… (More)

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