[Subtractive cDNA cloning and analysis of murine erythroid terminal differentiation related factor].

@article{Liu1999SubtractiveCC,
  title={[Subtractive cDNA cloning and analysis of murine erythroid terminal differentiation related factor].},
  author={Shufeng Liu and Zhu-qin Zhang and Jie Ma and Shou Feng Zhang and She-pu Xue},
  journal={Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae},
  year={1999},
  volume={21 2},
  pages={
          94-8
        }
}
OBJECTIVE Cloning and identification of cDNA related to erythroid terminal differentiation factor (MEDRF). METHODS cDNA related to erythroid terminal differentiation from the Friend virus anemia (FVA) infected splenic erythroblasts of BALB/c mice were performed by using subtractive hybridization combined with PCR technique. The splenic proerythroblasts isolated were cultured in the presence of erythropoietin for 36 hrs. Subtractive cDNA clones of differentially expressed in the 36 hrs… CONTINUE READING