[Subcellular re-distribution of F-actin in MCF7 cells upon inducible expression of CDKN2A/p16INK4a].

@article{Liang2017SubcellularRO,
  title={[Subcellular re-distribution of F-actin in MCF7 cells upon inducible expression of CDKN2A/p16INK4a].},
  author={Jianqing Liang and Manna Wang and Zhengrong Zhang and Long Yuan and You Zheng and Hongyan Huang and Qiang Sun and Jufang Wang and Xiaoning Wang},
  journal={Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology},
  year={2017},
  volume={33 4},
  pages={465-470}
}
Objective To investigate the effect of CDKN2A/p16INK4a, a cyclin-dependent kinase inhibitor, on cell morphology and cytoskeleton in MCF7 breast cancer cells by tetracycline operon (Tet-on) inducible gene expression system. Methods MCF7 cells, genetically null for p16INK4a expression, were sequentially infected with two viruses of Tet-on systems to make MCF7-pTet-on-p16INK4a cells, which were induced to express p16NK4a by doxycycline (Dox) treatment. Cell growth was evaluated by cell counting… CONTINUE READING