[Study on the properties of methyl parathion hydrolase from Pseudomonas sp. WBC-3].

@article{Chu2003StudyOT,
  title={[Study on the properties of methyl parathion hydrolase from Pseudomonas sp. WBC-3].},
  author={Xiaona Chu and Xianen Zhang and Yali Chen and Hong Liu and Donglin Song},
  journal={Wei sheng wu xue bao = Acta microbiologica Sinica},
  year={2003},
  volume={43 4},
  pages={453-9}
}
A methyl parathion degradation enzyme, or methyl parathion hydrolase (MPH, EC 3.1.8.1), locating in the soluble intracellular fraction of Pseudomonas sp. WBC-3, was purified 49.1-fold to homogeneity by one-step ion exchange chromatography. The physical and chemical properties of the purified MPH were studied. The purified MPH displayed relatively broad optimal temperature around 40 degrees. The activity of MPH was affected by pH and the optimal pH was 11.0. Cd2+ and Fe2+ could enhance the… CONTINUE READING

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