[Study on the expression of E2 gene of classical swine fever virus in Pichia pastoris and the immunological activity of its expression product].

Abstract

E2 gene of classical swine fever virus (CSFV) was cloned into secretory pPIC9K Pichia pastoris expression vector. After being linearized by digestion, the vector was transformed into Pichia pastoris by electroporation to integrate with the genome, the transformants with high copies were screened by G418 and were induced to express with methonal. The results… (More)

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Cite this paper

@article{Han2002StudyOT, title={[Study on the expression of E2 gene of classical swine fever virus in Pichia pastoris and the immunological activity of its expression product].}, author={Xue-qing Han and Xiangtao Liu and Yong Zhang and Qing-ge Xie and Bo Tian}, journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology}, year={2002}, volume={18 2}, pages={208-11} }