[Study on ribotyping of Lyme borreliosis spirochete in Guizhou province].

  title={[Study on ribotyping of Lyme borreliosis spirochete in Guizhou province].},
  author={Ding-Ming Wang and Qin Hao and Xing-he Cai and Kang-lin Wan and Zhao-xiao Wang and Jianchu Chen},
  journal={Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi},
  volume={24 12},
OBJECTIVE To define the main genotypes in Guizhou agricultural areas by molecular epidemiologic investigation of 21 Borrelia burgdorferi sensu lato of Lyme disease spirochetes and to provide the scientific bases for formulating a preventive policy. METHODS Polymerase chain reaction (PCR) technique was used to amplify the 23S(rrl)-5S(rrf) intergenic spacer, and amplified products were analyzed by restriction fragment length polymorphism (RFLP) and nucleotide sequencing. RESULTS There were… CONTINUE READING


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