[Study on fetal SRY gene in maternal plasma using nested polymerase chain reaction].

@article{Hong2006StudyOF,
  title={[Study on fetal SRY gene in maternal plasma using nested polymerase chain reaction].},
  author={Ping Hong and Pei-yuan Zhu and Yu-Feng Huang and Jian-feng Luan},
  journal={Zhonghua nan ke xue = National journal of andrology},
  year={2006},
  volume={12 4},
  pages={333-6}
}
OBJECTIVE To develop a nested polymerase chain reaction (PCR) technique for fetal SRY gene identification using cell-free fetal DNA in maternal plasma. METHODS Peripheral blood samples were obtained from 30 pregnant women and cell-free DNA was extracted by the phenol/chloroform method from plasma. The nested PCR was carried out to amplify the fragment of SRY gene by two sets of PCR primer pairs. Direct sequencing analysis was then performed on the PCR product. RESULTS Among the 17 women… CONTINUE READING
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