[Study on culture and in vitro osteogenesis of blood-derived human mesenchymal stem cells].

@article{Cao2005StudyOC,
  title={[Study on culture and in vitro osteogenesis of blood-derived human mesenchymal stem cells].},
  author={Cong Cao and Yinghai Dong and Yuqi Dong},
  journal={Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery},
  year={2005},
  volume={19 8},
  pages={642-7}
}
OBJECTIVE To establish a method of isolating and culturing adult human blood-derived stem cells(MSCs) and to investigate their osteogenic potential in vitro. METHODS Thirty peripheral blood collected from 30 adult volunteers (15 ml per person). Adult human MSCs derived from peripheral blood from the lymphocyte separation fluid fraction of mononuclear cells, cultured in alpha-Modified Eagle's Medium glucose containing 20% fetal bovine serum, and proliferated through a process of subculturing… CONTINUE READING
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