[Study of affinity modification kinetics as an approach to demonstrating cooperativity between substrate-recognition centers of bicentric enzymes].


The dependence of the initial rate of the affinity labelling of phenylalanyl-tRNA synthetase from E. coli MRE-600 by an phenylalanyl-tRNA analog--N-Br-Ac-[14C]Phe-tRNAPhe against reagent concentration was obtained. The curve runs through maximum therefore it is impossible to describe it by the traditional Kitz and Wilson scheme. The experimental results… (More)