[Studies on simultaneous detection of four foodborneviruses by multiplex reverse transcription-polymerase chain reaction].

Abstract

OBJECTIVE To establish a method for simultaneous detection of norovirus (NV), rotavirus (RV), astrovirus (AV) and hepatitis A virus (HAV) by multiplex reverse transcription-polymerase chain reaction (RT-PCR). METHODS Specific primers of the four viruses were designed based on the high conserved sequences, the reaction system and conditions optimized and… (More)

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