[Stable expression of Mycobacterium tuberculosis protein EspB in RAW264.7].

Abstract

OBJECTIVE We established a cell line stably expressing Mycobacterium tuberculosis secretory protein EspB in order to provide evidences for studying EspB in modulating the functions of macrophage. METHODS The recombinant plasmid pEGFP-C1-EspB was first constructed, then RAW264.7 cell was transfected with pEGFP-C1-EspB and pEGFP-C1 by liposome respectively… (More)

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