[Simultaneous determination of six Salvia miltiorrhiza gradients in rat plasma and brain by LC-MS/MS].

@article{Liu2014SimultaneousDO,
  title={[Simultaneous determination of six Salvia miltiorrhiza gradients in rat plasma and brain by LC-MS/MS].},
  author={Sheng-Min Liu and Zhi-hong Yang and Xiao-Bo Sun},
  journal={Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica},
  year={2014},
  volume={39 9},
  pages={1704-8}
}
To develop a LC-MS/MS method for the determination of protocatechuic acid, protocatechuic aldehyde, salvianolic acid A, salvianolic acid B, cryptotanshinone and tanshinone II(A) in rat plasma and brain. The plasma and brain samples were precipitated with ethyl acetate, then were separated on an Agilent eclipse plus-C18 column (2.1 mm x 50 mm, 3.5 microm) using acetonitrile (consisting of 0.1% formic acid) and water (consisting of 0.1% formic acid) as mobile phase in gradient elution mode. The… CONTINUE READING