[Simultaneous determination of four alisols in Rhizoma Alismatis by RP-HPLC].

@article{Luo2010SimultaneousDO,
  title={[Simultaneous determination of four alisols in Rhizoma Alismatis by RP-HPLC].},
  author={Zhangyan Luo and Aicun Zhou and Chao Zhang and Mian Zhang},
  journal={Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica},
  year={2010},
  volume={35 24},
  pages={3306-9}
}
OBJECTIVE To develop an HPLC method for the determination of alisol A, alisol F, alisol A 24-acetate and alisol B 23-acetate in the rhizome of Alisma orientalis. METHOD The separation was performed on a Shim-Pack CLC-ODS a C18 column (6 mm x 150 mm, 5 microm) eluted with the mobile phases of acetonitrile (A)-water (B) in gradient elution. The absorbance was monitored at 210 nm. Orthogonal test was adopted to optimize the extraction conditions of the four alisols. RESULT The correlation… CONTINUE READING