[Screening and identification of human anti-c-Met Fab from a phage antibody library].

@article{Sun2008ScreeningAI,
  title={[Screening and identification of human anti-c-Met Fab from a phage antibody library].},
  author={Hui Sun and Jia-yi Wan and Xiao-Juan Zhu and Yong-jun Jiao and Jin Zhu and Zhen-qing Feng and Zheng Hui Liu},
  journal={Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology},
  year={2008},
  volume={16 7},
  pages={505-8}
}
OBJECTIVE To screen anti-c-Met Fab from a phage antibody library and identify its binding activity. METHODS The expression of c-Met of HCC lines was identified by Western blot and immunofluorescence. Antibodies against c-Met were screened with immobilized antigen. After five rounds of panning, 30 randomly selected clones were identified by phage ELISA to select specific clones with high affinity. The positive clones were selected for Fab soluble expression in TOP10F and the binding activities… CONTINUE READING