[Reversible electric current generation by reconstituted mitochondrial transhydrogenase].


A direct measurement of the electrogenic activity of purified mitochondrial transhydrogenase has been carried out. For this purpose beef heart transhydrogenase was isolated and reconstituted with phospholipids to form proteoliposomes. The transhydrogenase proteoliposomes were incorporated into a membrane filter impregnated with a decane solution of… (More)