[Retrovirus-mediated SHP-1 gene expression in human breast cancer MDA-MB-231 cells].

OBJECTIVE To construct a retrovirus-mediated expression system carrying human SHP-1 gene to transfer SHP-1 gene in human breast cancer MDA-MB-231 cells. METHODS The full-length SHP-1 gene fragment was amplified by RT-PCR from the total RNA extracted from human breast cancer cell line MCF-7 over-expressing SHP-1 protein. The gene fragment was inserted into… (More)