[Research on construction of sheep lung adenomas virus pEGFP-C1/exJSRV-env and induction of malignant transformation in NIH3T3].

@article{Zhang2014ResearchOC,
  title={[Research on construction of sheep lung adenomas virus pEGFP-C1/exJSRV-env and induction of malignant transformation in NIH3T3].},
  author={Yu-fei Zhang and Yue Liu and Zhuan-Jia Wang and Xiao-lin Sun and Shu-ying Liu},
  journal={Bing du xue bao = Chinese journal of virology},
  year={2014},
  volume={30 3},
  pages={268-77}
}
This study aims to construct a eukaryotic expression system for envelope gene of Jaagsiekte sheep retrovirus, observes its localization in 293T cells, and investigates the potential in inducing malignant transformation of NIH3T3 cells. By RT-PCR, the full-length cDNA of envelope gene of Jaagsiekte sheep retrovirus (exJSRV-env) was amplified from the extract of naturally infected sheep lung. The clone of target gene was sub-cloned into eukaryotic expression system pEGFP-C1, and validated by PCR… CONTINUE READING