[Research on A23187 inducing HL-60 cells to differentiate into dendritic cells].


OBJECTIVE To investigate the method for calcium mobilization inducing rapidly to produce the dendritic cells (DC) from myeloid leukemia cells. METHODS HL-60 cells were cultured with calcium ionophore A23187 (45-720 ng/mL) or plus rhIFN-gamma (1000 U/mL) for 20-96 hours. The morphologic features of cells were observed under inverted microscope and scanning… (More)


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