[Recombination and identification of sense and antisense cyclinD1 eukaryotic expression vectors].

@article{Yan2003RecombinationAI,
  title={[Recombination and identification of sense and antisense cyclinD1 eukaryotic expression vectors].},
  author={Kexia Yan and Bingci Liu and Ming Xu and Baorong You and Ning Kang},
  journal={Wei sheng yan jiu = Journal of hygiene research},
  year={2003},
  volume={32 3},
  pages={180-2}
}
The exogenous gene cyclinD1 cDNA was inserted into eukaryotic expression vector pXJ41-neo by gene transduction and recombination techniques. The recombinant was detected by electrophoresis. Results showed that the recombinant sense plasmid (pXJ41-cyclinD1) was digested by HindIII into 229 bp, 427 bp and 7554 bp; while the recombinant antisense plasmid was digested into 427 bp, 1184 bp and 6599 bp, implying that sense and antisense pXJ41-cyclinD1 eukaryotic expression vectors were successfully… CONTINUE READING