Corpus ID: 38645435

[Real-time multiplex PCR for rapid detection of enteroviruses, adenoviruses and hepatitis A virus in clinical specimens].

@article{Oksanich2007RealtimeMP,
  title={[Real-time multiplex PCR for rapid detection of enteroviruses, adenoviruses and hepatitis A virus in clinical specimens].},
  author={A. Oksanich and E. Faizuloev and A. Nikonova and V. Kashirin and V. Lotte and O. Ivanova and V. Zverev},
  journal={Zhurnal mikrobiologii, epidemiologii, i immunobiologii},
  year={2007},
  volume={5},
  pages={
          65-70
        }
}
Real-time multiplex polymerase chain reaction (PCR) with internal positive control (IPC) was developed for simultaneous detection of adenoviruses (AV), enteroviruses (EV) and hepatitis A virus (HAV). Primes and probes labeled with different fluorophores (FAM, R6G, ROX, and Cy5) and able to detect up to four viral RNAs (DNAs) with high specificity in a single tube in real-time PCR were designed. Sensitivity and specificity of the method was estimated using cultural strains of 8 serotypes of EV… Expand
High frequency of false‐positive signals in a real‐time PCR‐based “Plus/Minus” assay