[Rapid isolation and characteristics analysis of microsatellites from Lutjanus russelli].

@article{Guo2007RapidIA,
  title={[Rapid isolation and characteristics analysis of microsatellites from Lutjanus russelli].},
  author={Y C Guo and Zhong-Duo Wang and Chu-Wu Liu and Yun Liu},
  journal={Yi chuan = Hereditas},
  year={2007},
  volume={29 3},
  pages={355-9}
}
The (CA)n DNA sequences in Lutjanus russelli were isolated through PCR arrays, and the characteristics of the microsatellite were analyzed. DNA was extracted from a sample of Lutjanus russelli, and digested with Hae+Dra. The fragments were ligated to pUCm-T vector and transferred into DH5alpha to construct a genomic library. The positive clones were isolated with universal M13 reverse and forward primers, M13 forward primer and the simple sequence repeats (SSRs) probes (CA)15, M13 reverse… CONTINUE READING