[Purification of recombinant H-NS protein of Yersinia pestis and characterization of its DNA-binding activity].

Abstract

OBJECTIVE The regulator protein H-NS of Yersinia pestis was expressed using the Escherichia coli BL21lambdaDE3 protein expression system, and its DNA-binding activity was characterized. METHODS The entire coding region of the hns gene was amplified by PCR from Y. pestis strain 201, and then cloned into the BamHI and Sal sites of the vector pET28a. The… (More)

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