[Purification of cadmium ion binding metallothionein-3 by proteinase digestion on affinity chromatographic column].


The gene encoding human metallothionein-3 (hMT-3) was synthesized and inserted into the poly-cloning sites of fusion expression vector pALEX, and fused downstream to its glutathione S-transferase (GST) fusion partner. Fusion protein GST-Cd2+-hMT-3 was expressed after isopropyl-beta-D-thiogalactopyranoside (IPTG) induction and addition of 0.1 mmol/L CdSO4… (More)


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