[Purification of an extracellular thiol-dependent hemolysin from Bacillus alvei].

@article{Alouf1977PurificationOA,
  title={[Purification of an extracellular thiol-dependent hemolysin from Bacillus alvei].},
  author={Joseph E. Alouf and Martine Kiredjian and C Geoffroy},
  journal={Biochimie},
  year={1977},
  volume={59 3},
  pages={329-36}
}
Alveolysin a sulfhydryl-dependent cytolytic extracellular protein released by Bacillus alvei has been purified by salting-out by ammonium sulfate, gel filtration, isoelectric focusing on pH gradient and chromatography on DEAE-cellulose. The purified protein after reduction by thiols (active hemolytic form) proved homogeneous by disc polyacrylamide gel… CONTINUE READING