Corpus ID: 40741715

[Purification and properties of chitinase from Enterobacter aerogenes].

  title={[Purification and properties of chitinase from Enterobacter aerogenes].},
  author={Y. Tang and J D Zhao and S Ding and Shaojun Liu and Z. Yang},
  journal={Wei sheng wu xue bao = Acta microbiologica Sinica},
  volume={41 1},
  • Y. Tang, J. Zhao, +2 authors Z. Yang
  • Published 1 February 2001
  • Chemistry, Medicine
  • Wei sheng wu xue bao = Acta microbiologica Sinica
A bacterium producing chitinase was isolated from the dead body of Gymephorap ruoergensis. A chitinase was isolated from the culture of E. aerogenes and purified by means of ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-100 column gel filtration. The purified chitinase showed homogeneity on the native polyacrylamide gel electrophoresis. Its molecular weight was estimated to be about 42.5 kD by SDS-PAGE. The optimum pH and temperature for hydrolysis of… Expand
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