Corpus ID: 35037214

[Purification and characterization of lipases from wheat germ].

  title={[Purification and characterization of lipases from wheat germ].},
  author={V. S. Kapranchikov and N. A. Zherebtsov and T. N. Popova},
  journal={Prikladnaia biokhimiia i mikrobiologiia},
  volume={40 1},
A method of isolation and purification of lipase (EC from the germ of wheat (Triticum aestivum) is described. Electrophoretically homogeneous preparation of the enzyme (specific activity, 622.5 x x 10(-3) mumol/min per mg protein) was obtained after purification in 61 times. The molecular weight of the enzyme, determined by gel chromatography, was 143 +/- 2 kDa. The optimal conditions for the enzyme were 37 degrees and pH 8.0. Homogeneous preparation of the lipase exhibited high… Expand

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