[Promoting effect of thrombin on proliferation of bone marrow-derived mesenchymal stem cells and its mechanisms].

@article{Chen2014PromotingEO,
  title={[Promoting effect of thrombin on proliferation of bone marrow-derived mesenchymal stem cells and its mechanisms].},
  author={Jin Chen and Yu-jie Ma and Zi Cheng Wang and Shan-Shan Lin and Feng-jun Xiao and Hua Wang and Li-Shing Wang and Zi-kuan Guo},
  journal={Zhongguo shi yan xue ye xue za zhi},
  year={2014},
  volume={22 2},
  pages={485-90}
}
This study was aimed to investigate the growth-promoting activity of thrombin on mesenchymal stem cells (MSC) and its mechanisms. Human bone marrow MSC were cultured in serum-free medium supplemented with graded concentrations of thrombin, and the proliferation status of MSC was detected by MTT test. The expression levels of protease-activated receptors (PAR) and c-MYC gene were detected by PCR. Activated Akt signaling pathway was revealed by Western blot, and specific inhibitors of the… CONTINUE READING