[Prokaryotic expression of N-terminal antigenic domain of duck plague virus gB protein and the establishment of putative indirect ELISA assay].

@article{Pan2008ProkaryoticEO,
  title={[Prokaryotic expression of N-terminal antigenic domain of duck plague virus gB protein and the establishment of putative indirect ELISA assay].},
  author={Huaqi Pan and Rui-bing Cao and Lei Liu and Hailiang Sun and Xiangbo Ji and Yongjun Chen and Puyan Chen},
  journal={Wei sheng wu xue bao = Acta microbiologica Sinica},
  year={2008},
  volume={48 1},
  pages={98-102}
}
Based on the antigenic analysis of duck plague virus (DPV) gB protein, we designed a pair of primers to amplify the gene fragment encoding high antigenic domain of DPV N-terminal gB protein from the DPV genome. The cloned gene was digested with EcoR I and Hind III and then inserted into pET32a vector to obtain the recombinant pET-gB1 plasmid. The recombinant plasmid was transformed into E. coli BL21, and expressed in very high level after induced with IPTG. The expressed product was analyzed by… CONTINUE READING