[Problems associated with the use of highly degenerated oligonucleotide probes. Identification of mRNA and cDNA clones corresponding to the gene for the alpha subunit of Na+,K+-ATPase].

Abstract

Oligonucleotides deduced from the amino acid sequence of a hexapeptide Lys-Asp-Phe-Ala-Glu-Asn were synthesized and used as probes to screen a pig kidney cDNA library for a specific DNA sequence coding for the alpha-subunit of Na+, K+-ATPase. It was shown that the mixed oligoprobe, consisting of 64 heptadecamers, could be only suitable for mRNA blot… (More)

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@article{Petrukhin1985ProblemsAW, title={[Problems associated with the use of highly degenerated oligonucleotide probes. Identification of mRNA and cDNA clones corresponding to the gene for the alpha subunit of Na+,K+-ATPase].}, author={Konstantin Petrukhin and A. V. Grishin and Sergei Arsenian and Natalia E. Broude and Vitaly Grinkevich}, journal={Bioorganicheskaia khimiia}, year={1985}, volume={11 12}, pages={1636-41} }