[Primary culture and identification of rat glomerular podocytes].

Abstract

OBJECTIVE To establish an easy and feasible method for primary culture and identification of rat glomerular podocytes. METHODS Glomeruli from Sprague-Dawley (SD) rats weighing 60-100 gram were isolated by the method of different size combination of screen. Isolated glomeruli were appropriately digested with 2 g/L type IV collagenase and cultured in 25 cm2… (More)

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